ENCAB000BDY
Antibody against Homo sapiens POU5F1
Homo sapiens
K562, MCF-7
partially characterized
Homo sapiens
GM12878, HeLa-S3, HepG2, IMR-90, heart
not characterized to standards
- Status
- released
- Source (vendor)
- Cell Signaling
- Product ID
- 2750S
- Lot ID
- 2
- Characterized targets
- POU5F1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide corresponding to amino acids near the carboxy terminus of human Oct-4.
- External resources
Characterizations
POU5F1 (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody 2750S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 45.0.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- #1141K562 POU5F1(2750s).jpg
POU5F1 (Homo sapiens)
MCF-7
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody 2750S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 45.0.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 11.jpg
POU5F1 (Homo sapiens)
HepG2
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody 2750S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 45.0.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 12.jpg
POU5F1 (Homo sapiens)
GM12878
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody 2750S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 45.0.
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- expt1065WB-033115_1_Oct-4-2750S.jpg
POU5F1 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
not compliant
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, HepG2, IMR90 and Heart using the antibody 2750S. Molecular mass: 38571 Da
- Reviewer comment
- Band is either missing or is not 50% of signal
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- POU5F1_2750S_WB_a.jpg
POU5F1 (Homo sapiens)
HepG2
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 2750S .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- No visible band that isn't also in the IgG lane. Expected size not given, but would not be compliant anyway.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- POU5F1_2750S_WB_b.jpg