ENCAB000BDY

Antibody against Homo sapiens POU5F1

Homo sapiens
MCF-7, K562
partially characterized
Homo sapiens
HepG2, IMR-90, HeLa-S3, heart, GM12878
not characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
2750S
Lot ID
2
Characterized targets
POU5F1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide corresponding to amino acids near the carboxy terminus of human Oct-4.
External resources

Characterizations

POU5F1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody 2750S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 45.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
POU5F1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7 using the antibody 2750S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 45.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
POU5F1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2 using the antibody 2750S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 45.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
POU5F1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878 using the antibody 2750S. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 45.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
POU5F1 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
Method: immunoblot
Attachment from submitter
not compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, HepG2, IMR90 and Heart using the antibody 2750S. Molecular mass: 38571 Da
Reviewer comment
Band is either missing or is not 50% of signal
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
POU5F1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 2750S .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
No visible band that isn't also in the IgG lane. Expected size not given, but would not be compliant anyway.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford