1. Antibodies
  2. Homo sapiens
  3. NEUROD1

ENCAB000BDX

Antibody against Homo sapiens NEUROD1

Homo sapiens
K562, MCF-7
partially characterized
Homo sapiens
at least one cell type or tissue
not pursued
Homo sapiens
GM12878, HeLa-S3, HepG2, IMR-90, heart
not characterized to standards
Status
released
Source (vendor)
Cell Signaling
Product ID
4373S
Lot ID
1
Characterized targets
NEUROD1 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide surrounding Gln15 of human NeuroD protein.
External resources

Characterizations

NEUROD1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody 4373S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Band of expected size looks week in input and though stronger in IP than IgG, it is subsumed by the signal of the heavy chain so it's tough to call.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 10.jpg
NEUROD1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody 4373S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Multiple bands but band of expected size is >50% of the total signal in the lane.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 9.jpg
NEUROD1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody 4373S. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 49.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
NEUROD1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody 4373S. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 49.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 NEUROD1.JPG
NEUROD1 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
Method: immunoblot
Attachment from submitter
Attachment from submitters
not compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, HepG2, IMR90 and Heart using the antibody 4373S. Molecular mass: 39920 Da
Reviewer comment
Band is either missing or is not within 20% of expected size (lanes 5 and 6)
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
NEUROD1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody 4373S .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~49 kDa.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford