1. Antibodies
  2. Homo sapiens
  3. RUNX1

ENCAB000BDQ

Antibody against Homo sapiens RUNX1

Homo sapiens
GM12878, K562, MCF-7, liver
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
HepG2, HeLa-S3
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-365644
Lot ID
K0612
Characterized targets
RUNX1 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
affinity
Isotype
IgG1
Antigen description
Amino acids 186-250 mapping within an internal region of RUNX1 of human origin
External resources

Characterizations

RUNX1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-365644. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 52.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
RUNX1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-365644. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
 8.jpg
RUNX1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target RUNX1 is represented by the attached position weight matrix (PWM) derived from ENCFF029TIT. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab). Accept probability score: 0.964710053054 Global enrichment Z-score: 6.23936631556 Positional bias Z-score: 8.87946202522 Peak rank bias Z-score: 5.47725783349 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
RUNX1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-365644. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 52.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
RUNX1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-365644.
Reviewer comment
Multiple, defuse bands not >50% of total signal near expected size (52kD)
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
RUNX1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-365644 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size 48-50 KDa.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford