ENCAB000BCZ
Antibody against Homo sapiens SKI
Homo sapiens
K562, HepG2, HEK293T
partially characterized
Homo sapiens
at least one cell type or tissue
not pursued
Homo sapiens
GM12878, HeLa-S3, MCF-7, liver
not characterized to standards
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-9140
- Lot ID
- F2712
- Characterized targets
- SKI (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Epitope corresponding to amino acids 400-728 mapping at the C-terminus of Ski of human origin
- External resources
Characterizations
SKI (Homo sapiens)
K562
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-9140. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- 1060_10_SKI_sc-9140.jpg
SKI (Homo sapiens)
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody sc-9140. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 80(95-115)
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- expt1063_2-Ski (H-329)-sc-9140.jpg
SKI (Homo sapiens)
MCF-7
not compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-9140. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- Too many bands, hard to tell which one is correct
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- expt1066-5-Ski (H-329)-sc-9140.jpg
SKI (Homo sapiens)
not submitted for review by lab
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-9140. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 80(95-115)
- Submitted by
- Denis Salins
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- Expt1089_6-Ski (H-329)-sc-9140-1.jpg
SKI (Homo sapiens)
HEK293T
compliant
- Caption
- Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody sc-9140. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 80(95-115)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- Expt1124_1-SKI-sc-9140.JPG
SKI (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
compliant
- Caption
- a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-9140. Molecular mass: 80005 Da
- Reviewer comment
- Multiple bands, none >50 of total signal in lane. Expected size (~80kD) not indicated.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SKI_sc-9140_WB_a.jpg
SKI (Homo sapiens)
HepG2
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-9140 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- No strong band. Also missing expected size and arrow, but even with those, wouldn't be compliant.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SKI_sc-9140_WB_b.jpg