1. Antibodies
  2. Homo sapiens
  3. RFX1

ENCAB000BCU

Antibody against Homo sapiens RFX1

Homo sapiens
K562, MCF-7
characterized to standards
Homo sapiens
GM12878, HepG2, HeLa-S3, liver
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-10650
Lot ID
H2108
Characterized targets
RFX1 (Homo sapiens)
Host
goat
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Epitope mapping within an internal region of RFX1 of human origin
External resources

Characterizations

RFX1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-10650. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
RFX1 (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
compliant
Caption
The motif for target RFX1 is represented by the attached position weight matrix (PWM) derived from ENCFF653BOL. Motif enrichment analysis was done by Dr. Zhizhuo Zhang (Broad Institute, Kellis Lab) using known motifs (http://compbio.mit.edu/encode-motifs/) and previously published ChIP-seq data (http://www.broadinstitute.org/~zzhang/motifpipeline/data/TrainSetInfo.txt). The accept probability score of the given transcription factor was calculated using a Bayesian approach. This analysis also includes three motif enrichment scores, computed by overlapping the motif instances with the given ChIP-seq peak locations, as well as an enrichment rank. For more information on the underlying statistical methods, please see the attached document. Accept probability score: 0.963986448 Global enrichment Z-score: 8.713755402 Positional bias Z-score: 12.96617763 Peak rank bias Z-score: 4.140652295 Enrichment rank: 1.0
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
RFX1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody sc-10650. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
The size conflicts with Uniprot, the gel is unreadable
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
RFX1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-10650. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 130.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 MCF7-RFX1.jpg
RFX1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
Attachment from submitter
Attachment from submitters
not compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-10650.
Reviewer comment
Multiple bands, none >50 of total signal in lane. Expected size (~130kD) not indicated.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
RFX1 (Homo sapiens)
HeLa-S3
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: HelaS3, using the antibody sc-10650 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~104 kDa.
Reviewer comment
Band not within 20% of expected size
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford