ENCAB000BCN

Antibody against Homo sapiens MTA1

Homo sapiens
K562
characterized to standards
Homo sapiens
MCF-7
characterized to standards with exemption
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
GM12878, HepG2, HeLa-S3, liver
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-10813
Lot ID
A1309
Characterized targets
MTA1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Epitope corresponding to amino acids 513-678 of MTA1 of human origin
External resources

Characterizations

MTA1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody sc-10813. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 80.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
MTA1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody sc-10813. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Multiple bands, none marked as major with at least 50% of the total signal in the IP lane. Might be acceptable with a rescue knockdown characterization.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
MTA1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody sc-10813. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
Download
MTA1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody sc-10813. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
Band shared by IgG lane.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
MTA1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody sc-10813. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 80.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
MTA1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody sc-10813, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
GATAD2A, GATAD2B and CHD4 have interactions with MTA1 with 2,3 and 5 publications on Biogrid. http://thebiogrid.org/114562/summary/homo-sapiens/mta1.html
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
MTA1 (Homo sapiens)
GM12878K562HepG2HeLa-S3MCF-7liver
Method: immunoblot
Attachment from submitter
not compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3,MCF7, Liver using the antibody sc-10813.
Reviewer comment
Multiple bands, none >50% of total signal in lane. Expected size (~80kD) not indicated.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
MTA1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: MCF7, using the antibody sc-10813 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~80 kDa.
Submitter comment
MTA1 has 3 isoforms, 81KD, 79KD and 49KD, it may explain the multiple bands.
Reviewer comment
Multiple bands and marked band does not comprise 50% of signal
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford