ENCAB000BBO
Antibody against Homo sapiens E2F2
Homo sapiens
GM12878, K562, HepG2, HeLa-S3, liver
not characterized to standards
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-633
- Lot ID
- H1412
- Characterized targets
- E2F2 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Epitope mapping at the C-terminus of E2F-2 of human origin
- External resources
Characterizations
E2F2 (Homo sapiens)
GM12878K562HepG2HeLa-S3liver
Method: immunoblot
not compliant
- Caption
- a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3, Liver using the antibody sc-633.
- Reviewer comment
- Multiple bands, none >50 of total signal in lane. Expected size (~47kD) not indicated.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- E2F2_sc-633_WB_a.jpg
E2F2 (Homo sapiens)
HeLa-S3
Method: immunoprecipitation
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HelaS3, using the antibody sc-633 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size is 47-48 kDa.
- Reviewer comment
- IgG shares same band
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- E2F2_sc-633_WB_b.jpg