1. Antibodies
  2. Homo sapiens
  3. CTBP1

ENCAB000BAY

Antibody against Homo sapiens CTBP1

Homo sapiens
GM12878, K562, HepG2, liver, MCF-7, HEK293T
characterized to standards
Homo sapiens
HeLa-S3
not characterized to standards
Status
released
Source (vendor)
Sigma
Product ID
C8741
Lot ID
065K4751
Characterized targets
CTBP1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide corresponding to amino acids 423-440 of human CtBP1, conjugated to KLH via an N-terminal added lysine residue.
External resources

Characterizations

CTBP1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody C8741. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
CTBP1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody C8741. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
CTBP1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of CTBP1 from K562 cells using C8741. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with C8741. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 48 kDa.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
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 CTBP1.jpeg
CTBP1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using C8741, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
CTBP1 (Homo sapiens)
GM12878K562HepG2HeLa-S3liver
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3, Liver 1 using the antibody C8741.
Reviewer comment
Is lane 4 warped? Not sure if that's a band or not. Expected size also not given (~48kD)
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
CTBP1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody C8741 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size 46-48 kDa.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
CTBP1 (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody C8741. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 48.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford