1. Antibodies
  2. Homo sapiens
  3. TARDBP

ENCAB000BAX

Antibody against Homo sapiens TARDBP

Homo sapiens
GM12878, K562, HepG2, liver, MCF-7, HEK293T
characterized to standards
Homo sapiens
HeLa-S3
not characterized to standards
Status
released
Source (vendor)
Sigma
Product ID
T1705
Lot ID
089K4758
Characterized targets
TARDBP (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide corresponding to amino acids 181-198 of human TDP-43 (GeneID: 23435), conjugated to KLH.
External resources

Characterizations

TARDBP (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody T1705. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 43.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
TARDBP (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody T1705. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 43.0
Reviewer comment
Overexposed but band looks to be there above the heavy chain.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
TARDBP (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody T1705. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 43.0
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
TARDBP (Homo sapiens)
HEK293T
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HEK293T, using the antibody T1705. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 43.0
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
TARDBP (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation of TDP43 from K562 cells using T1705. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with T1705. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. The expected band size is 43 kDa.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
TARDBP (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using T1705, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
TARDBP (Homo sapiens)
GM12878K562HepG2HeLa-S3liver
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, HepG2, HelaS3, Liver 1 using the antibody T1705.
Reviewer comment
Multiple bands, but major band near expected size (~43 kD).
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
TARDBP (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody T1705 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size is 44-45 kDa.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford