ENCAB000AZZ

Antibody against Homo sapiens TCF7L2

Homo sapiens
GM12878, K562
partially characterized
Homo sapiens
HeLa-S3, heart, HepG2, IMR-90, A549
not characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
MA514935
Lot ID
none
Characterized targets
TCF7L2 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide corresponding to residues surrounding Leu330 of human TCF4.
External resources

Characterizations

TCF7L2 (Homo sapiens)
GM12878K562HeLa-S3heartHepG2IMR-90A549
Method: immunoblot
Attachment from submitter
compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, Heart, HepG2, IMR90 and A549 using the antibody MA5-14935. Molecular mass: 67919 Da
Reviewer comment
Band is either missing (lanes 4 and 6) or is not 50% of signal (lanes 3, 5, and 7). Lanes 1 and 2 are compliant.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
TCF7L2 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody MA514935 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size: 67.9 kD
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford