ENCAB000AZS
Antibody against Homo sapiens SMAD1
Homo sapiens
GM12878, HepG2, A549
partially characterized
Homo sapiens
K562, HeLa-S3, heart, IMR-90
not characterized to standards
- Status
- released
- Source (vendor)
- Thermo Fisher
- Product ID
- PA5-17122
- Lot ID
- none
- Characterized targets
- SMAD1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide corresponding to residues surrounding Ser190 of human Smad1.
- External resources
Characterizations
SMAD1 (Homo sapiens)
GM12878K562HeLa-S3heartHepG2IMR-90A549
Method: immunoblot
compliant
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, Heart, HepG2, IMR90 and A549 using the antibody PA5-17122. Molecular mass: 52260 Da
- Reviewer comment
- Band is either too faint (lane 2) or is not 50% of signal (Lanes 3, 4, 6). Lanes 1, 5, and 7 are compliant
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SMAD1_PA5-17122_WB_a.jpg
SMAD1 (Homo sapiens)
HepG2
Method: immunoprecipitation
compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody PA5-17122 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Expected size ~52 kD
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- SMAD1_PA5-17122_WB_b.jpg