ENCAB000AZO
Antibody against Homo sapiens CTNNB1
Homo sapiens
GM12878, IMR-90, heart, A549
partially characterized
Homo sapiens
K562, HeLa-S3, HepG2
not characterized to standards
- Status
- released
- Source (vendor)
- Thermo Fisher
- Product ID
- PA5-16192
- Lot ID
- none
- Characterized targets
- CTNNB1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- Protein A
- Isotype
- IgG
- Antigen description
- Synthetic peptide (Blue Carrier-coupled) corresponding to the carboxy-terminus of human b-catenin.
- External resources
Characterizations
CTNNB1 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heartA549
Method: immunoblot
compliant
- Caption
- Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, HepG2, IMR90, Heart and A549 using the antibody PA5-16192. Molecular mass: 85497 Da
- Reviewer comment
- Band is either missing (lanes 2 and 3) or is not 50% of signal (lane 4). Lanes 1, 5, 6, and 7 are compliant).
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- CTNNB1_PA5-16192_WB_a.jpg
CTNNB1 (Homo sapiens)
GM12878
Method: immunoprecipitation
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878(redo in HepG2), using the antibody PA5-16192 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
- Reviewer comment
- Multiple prominent bands - may possibly be rescued with a knockdown characterization against the factor.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- CTNNB1_PA5-16192_WB_b.jpg
