ENCAB000AYU

Antibody against Homo sapiens BMI1

Homo sapiens
K562, HeLa-S3, heart, HepG2, IMR-90, GM12878
not characterized to standards
Status
released
Source (vendor)
Novus
Product ID
NBP1-96140
Lot ID
A-1
Characterized targets
BMI1 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
Protein G
Isotype
IgG2bκ
Antigen description
Human Bmi1 protein [Swiss-Prot# P35226]
External resources

Characterizations

BMI1 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
Method: immunoblot
Attachment from submitter
not compliant
Caption
a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, Hela S3, HepG2, IMR90, Heart using the antibody NBP1-96140.
Reviewer comment
Multiple bands, none >50% of total signal in lane. Expected size (~37kD) not indicated.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
BMI1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody NBP1-96140 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~37 kDa.
Reviewer comment
Band not within 20% of expected size
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford