ENCAB000AYU
Antibody against Homo sapiens BMI1
Homo sapiens
GM12878, K562, HeLa-S3, HepG2, IMR-90, heart
not characterized to standards
- Status
- released
- Source (vendor)
- Novus
- Product ID
- NBP1-96140
- Lot ID
- A-1
- Characterized targets
- BMI1 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Purification
- Protein G
- Isotype
- IgG2bκ
- Antigen description
- Human Bmi1 protein [Swiss-Prot# P35226]
- External resources
Characterizations
BMI1 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90heart
Method: immunoblot
not compliant
- Caption
- a) Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order: GM12878, K562, Hela S3, HepG2, IMR90, Heart using the antibody NBP1-96140.
- Reviewer comment
- Multiple bands, none >50% of total signal in lane. Expected size (~37kD) not indicated.
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- BMI1_NBP1-96140_WB_a.jpg
BMI1 (Homo sapiens)
K562
Method: immunoprecipitation
not compliant
- Caption
- b) Immunoprecipitation was performed on nuclear extracts from the cell line: K562, using the antibody NBP1-96140 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~37 kDa.
- Reviewer comment
- Band not within 20% of expected size
- Submitted by
- Trupti Kawli
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG006996
- Download
- BMI1_NBP1-96140_WB_b.jpg