ENCAB000AYH

Antibody against Homo sapiens SMAD1

Homo sapiens
GM12878, HeLa, K562
partially characterized
Homo sapiens
A549, IMR-90, HeLa-S3, HepG2, heart
not characterized to standards
Status
released
Source (vendor)
Rockland
Product ID
600-401-B29
Lot ID
22895
Characterized targets
SMAD1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
A phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein.
External resources

Characterizations

SMAD1 (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody 600-401-B29. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Submitter comment
The original decision of not compliant was overruled by the antibody review committee (3/15/16).
Reviewer comment
Band of expected size not present in input and is around the size of the heavy chain.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
SMAD1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody 600-401-B29. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 52.0.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
SMAD1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: K562 using the antibody 600-401-B29. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 52.0.
Reviewer comment
Tough call, as the immunoreactive signal at indicated size is weak but appears to slightly larger than the signal in the IgG lane.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
SMAD1 (Homo sapiens)
GM12878K562HeLa-S3HepG2IMR-90A549heart
Method: immunoblot
Attachment from submitter
not compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, HepG2, IMR90, A549 and Heart using the antibody 600-401-B29. Molecular mass: 52260 Da
Reviewer comment
Band is missing
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
SMAD1 (Homo sapiens)
HeLa
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: Hela, using the antibody 600-401-B29 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size ~52 kDa.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford