ENCAB000AYC

Antibody against Homo sapiens ARNT

Homo sapiens
GM12878
partially characterized
Homo sapiens
K562, HeLa-S3, heart, HepG2, IMR-90, A549
not characterized to standards
Status
released
Source (vendor)
Thermo Fisher
Product ID
PA1-16510
Lot ID
none
Characterized targets
ARNT (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.
External resources

Characterizations

ARNT (Homo sapiens)
GM12878K562HeLa-S3heartHepG2IMR-90A549
Method: immunoblot
Attachment from submitter
not compliant
Caption
Western blot analysis of nuclear lysates prepared from multiple cells lines loaded in the order : GM12878, K562, HelaS3, Heart, HepG2, IMR90 and A549 using the antibody PA1-16510. Molecular mass: 86636 Da
Reviewer comment
Band is either missing or is not 50% of signal
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford
ARNT (Homo sapiens)
GM12878
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
b) Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody PA1-16510 .The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG. Expected size 84-86 kDa.
Submitted by
Trupti Kawli
Lab
Michael Snyder, Stanford