ENCAB000AUR
Antibody against Homo sapiens H3K9me3
Bos taurus
any cell type or tissue
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab8898
- Lot ID
- GR36126-3
- Characterized targets
- H3K9me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 402
- External resources
Characterizations
H3K9me3 (Homo sapiens)
thymus
Method: immunoblot
compliant
- Submitter comment
- This antibody was used in a pair with a second antibody. No characterization was done for this antibody. The vendor immunoblot is being provided.
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K9me3 (Homo sapiens)
Method: ChIP-seq comparison
exempt from standards
- Caption
- This validation relies on the use of antibodies to a histone modification (H3K9me3) in in two related cell types (H1 derived neurons and bipolar spindle neurons), demonstrating highly similar patterns of enrichment are obtained with each antibody. Please note that the two antibodies originate from two lots of the same vendor antibody part number. The first track shown used an antibody to H3K9me3 previously characterized to Encode standards (PchAb 1259, ENCAB369JSU, bipolar spindle neurons), and the second track shown used an antibody to H3K27me3 (PchAb 402, ENCAB000AUR, H1 derived neurons). Please note, the genomewide correlation of the following two tracks is slightly lower than the regular standard but the tracks were obtained from two related cell types, rather than from a single cell type. There are not data available to us to attempt the ChIP-ChIP comparison in a single cell type.
- Submitter comment
- We have supplied a compliant western blot to the DCC in which it was used in thymus cells [visible from the above hyperlink]. For secondary validation, we have submitted a ChIP-ChIP comparison analysis of this antibody with another H3K9me3 antibody already characterized to standards. The analysis shows highly similar patterns of enrichment obtained with each antibody, but the genomewide correlation is slightly lower than the standard due to the relatively limited coverage of H3K9me3 in non-repetitive regions of the genome compared to other more ubiquitously occurring peaks. We are asking for the secondary validation to be exempted from standard for this reason. We have no remaining inventory of the antibody in our lab.
- Reviewer comment
- PJF: I agree that the ChIPseq comparison is appropriate for this secondary validation.
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- H3K9me3 PchAb 402 SAV.pdf