1. Antibodies
  2. influenzavirus

ENCAB000ASV

Antibody against influenzavirus HA

influenzavirus
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Covance
Product ID
MMS-101P
Lot ID
unknown
Characterized targets
HA (influenzavirus)
Host
mouse
Clonality
monoclonal
Antigen description
Clone 16B12 was raised against the twelve amino acid peptide CYPYDVPDYASL. This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
Antigen sequence
CYPYDVPDYASL

Characterizations

HA (influenzavirus)
Method: immunoprecipitation
Attachment PDF Icon
not reviewed
Caption
An IP western was performed using GM12878, HeLa and K562 nuclear extract. For each extract, lane 1 is nuclear extract, lane 2 is the immunoprecipitated sample, and lane 3 is the sample immunoprecipitated with a control rabbit IgG. Due to the extremely low level of E2F1 in most cells, it is hard to detect by western prior to IP. However, for all three cell types, the E2F1 antibody immunoprecipitated one major band of the right size that was not precipiated by a control rabbit IgG.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
HA (influenzavirus)
Method: ChIP-seq comparison
Attachment PDF Icon
not reviewed
Caption
ChlPseq was performed in Hela cells stably trnsfected with a plasmid expressing an HA tagged E21 protein. Two different biological replicates of HeLa cells were used; one sample was ChIPseq profiles: 99% of the top 40% of targets identified with the HA antibody are in teh targets identified using the E2F1 antibody and 97.5% of the top 40% of targets identified using the E2F1 antibody are in the target identified using the HA antibody.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford