1. Antibodies
  2. Homo sapiens
  3. ATF2

ENCAB000ASU

Antibody against Homo sapiens ATF2

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-81188
Lot ID
H0609
Characterized targets
ATF2 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
crude
Isotype
IgG
Antigen description
Raised against a recombinant protein corresponding to an internal region of ATF2 of human origin.

Characterizations

ATF2 (Homo sapiens)
Method: immunoblot
Attachment from submitter
Attachment from submitters
not reviewed
Caption
Western blot protocol: Whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size (~70 kDa) is visible, representing strongest signal in the lane. Figure legend: IP-western with sc-81188 in whole cell lysate (WCL) of K562 and HepG2; PM=protein marker. ATF2 band is indicated.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
ATF2 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Caption
As per ENCODE data standards, all SEQUEST results are attached (ENCODE_HAIB_ATF2_sc81188_08252011_MassSpec.pdf), including common contaminants. Target protein is listed as hit 5 in the ~70 kDa band.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB