ENCAB000ASS

Antibody against Homo sapiens ATF2

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Cell Signaling
Product ID
9221
Lot ID
unknown
Characterized targets
ATF2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
Protein A
Antigen description
A synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2.

Characterizations

ATF2 (Homo sapiens)
Method: immunoprecipitation
not reviewed
Caption
pT71-ATF2 ChIP-Seq analysis supports successful ChIP. A 7% of MACS-called pT71-ATF2 ChIP-Seq peaks (p-value <1e-10) are found within 1000bp of a known TSS.B pT71-ATF2 peaks preferentially overlap with the transcription factorsATF1. ATF2, CREB and JUN. C,D De novo motif discovery with the MEME suite of analysis software using 100bp of sequence flanking the top 1000 pT71-ATF2 peaks identifies the TRE (p-value < 8.85e-5) and CRE (p-value < 1.27e-3) motifs as enriched DNA elements. 32% of pT71-ATF2 peaks are associated with a TRE (p-value < 0.0001) relative to 4.6% of non-redundant RefSeq TxStart sites (p-valuebonf < 3e-300), and 12% of pT71-ATF2 peaks are associated with a CRE relative to 8.6% of non-redundant RefSeq TxStart sites (p-valuebonf < 7e-22). pT71-ATF2 peak maxima and motif position are coincident.
Submitted by
Kevin Struhl
Lab
Michael Snyder, Stanford
ATF2 (Homo sapiens)
Method: immunoblot
not reviewed
Caption
Image would not load. Please contact me for validation 1 image. Western blot analysis identifies one major antigen at the predicted size of ~70kD. The pT71-ATF2 antibody identifies an antigen that is inducible in response to stress such as serum starvation while the ATF2 antibody (sc-6233) shows no change in the amount of ATF2 present.
Submitted by
Kevin Struhl
Lab
Michael Snyder, Stanford