ENCAB000ASG
Antibody against Homo sapiens H3K4me3
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Millipore
- Product ID
- 07-473
- Lot ID
- R0903B0054
- Characterized targets
- H3K4me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Synthetic peptide corresponding to residues surrounding and including trimethlyated Lys 4 of Histone H3.
- Aliases
- bradley-bernstein:PchAb 103-V
- External resources
Characterizations
H3K4me3 (Homo sapiens)
K562
Method: immunoblot
compliant
- Caption
- Western blot analysis of K562 lysate using rabbit polyclonal to H3K4me3. Submitting lab has confirmed with the vendor that lots R0903B0054, DAM1619901, DAM1623866, DAM1644057, DAM1651667, and DAM1661080 are identical.
- Submitter comment
- M Hatan reports lab confirmed with vendor that these lots are identical and should require only one characterization: DAM1619901, DAM1623866, DAM1644057, DAM1651667, DAM1661080, R0903B0059
- Submitted by
- Meital Hatan
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K4me3 (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- Suitably modified peptides modeled on histone tails were obtained from Abcam.Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad) Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray.Place on rocker for 30 minutes. Dump off SuperBlock. Submitting lab has confirmed with the vendor that lots R0903B0054, DAM1619901, DAM1623866, DAM1644057, DAM1651667, and DAM1661080 are identical.
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570