ENCAB000ASD

Antibody against Homo sapiens H3K27me3

Homo sapiens
any cell type or tissue
characterized to standards with exemption
Status
released
Source (vendor)
Millipore
Product ID
07-449
Lot ID
DAM1662421
Characterized targets
H3K27me3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
Protein A
Isotype
IgG
Antigen description
KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...AR(me3K)SAP... in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.
Aliases
bradley-bernstein:PchAb 69-V
External resources

Characterizations

H3K27me3 (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
This validation relies on the use of antibodies to a histone modification (H3K27me3) in ACC112 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. Please note that the two antibodies originate from two lots of the same vendor antibody part number. The first track shown used an antibody to H3K27me3 (PchAb 69-V, ENCAB000ASD), and the second track shown used an antibody to H3K27me3 previously characterized to Encode standards (PchAb 683, ENCAB000BLA).
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
H3K27me3 (Homo sapiens)
K562
Method: immunoblot
Attachment from submitter
exempt from standards
Caption
This antibody lot did not have a primary completed for it. However, it was used in an experiment where each replicate used a different lot. This characterization is for lot ENCAB000BLA. It was used with this lot in experiment ENCSR475NLR.
Submitter comment
This is a request for exemption as the first replicate was done as part of ENCODE2.
Reviewer comment
These experiments with duplicate antibodies were considered valid if they passed replicate concordance.
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad