ENCAB000AQF
Antibody against Homo sapiens H3K36me3
Bos taurus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab9050
- Lot ID
- 761748
- Characterized targets
- H3K36me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, tri methylated at K36.
- Aliases
- bradley-bernstein:PchAb 80-V
- External resources
Characterizations
H3K36me3 (Homo sapiens)
thymusthymus
Method: immunoblot
compliant
- Submitter comment
- This western blot demonstrates the antibody recognizes a protein of the molecular weight consistent with human histone H3
- Reviewer comment
- Though this western blot was carried out with calf biosamples, the ENCODE Antibody Review Panel has reviewed this characterization and allowed this lot to be exempted for use in human biosamples on November 11, 2016.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K36me3 (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- We performed a dot blot analysis using the large set of modified peptides provided in the Active Motif MODified Histone Peptide Array, catalog #13005. The principal peptide recognized by this H3K36me3 antibody bore the correct modification. An acceptably low amount of cross reactivity was observed involving a few other methylated lysine residues, principally on H4K20.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991