ENCAB000APE
Antibody against Homo sapiens H3K27ac
Homo sapiens
any cell type or tissue
not characterized to standards
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab4729
- Lot ID
- 509313
- Characterized targets
- H3K27ac (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, acetylated at K27.
- Aliases
- bradley-bernstein:PchAb 66-V
- External resources
Characterizations
H3K27ac (Homo sapiens)
K562
Method: immunoblot
not reviewed
- Caption
- done by Bing Ren's lab- taken from http://compbio.med.harvard.edu/antibodies/system/images/77/original/Slide12.JPG?1291573588
- Reviewer comment
- KCO: This is fairly passable, but it is preferred to have an immunoblot image that labels what cell line you’re using (stated under Antibody Characterizations Standards, August 2015)
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K27ac (Homo sapiens)
Method: dot blot assay
not compliant
- Caption
- Not Specific (40%); Cross-reacting peptides: H3K18ac (30%), H3K9ac (20%), H4K5ac (10%)\n\nDone by Bing Ren's lab- taken from:\nhttp://compbio.med.harvard.edu/antibodies/system/images/77/original/Slide12.JPG?1291573588
- Reviewer comment
- Too much cross-reactivity, not enough details about the image, caption.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991