ENCAB000AOA

Homo sapiens

at least one cell type or tissue

awaiting characterization

Method: immunoprecipitation

not reviewed

Caption: TCF7L2 ChIP-seq peak overlap comparison. Two different TCF7L2 ChIP-seq datasets were prepared from HelaS3 cells either using the anti-TCF7L2 C9B9 antibody or the C48H11 antibody. Peaks were called using Solesearch peak calling program and the lists were compared directly.
Submitted by: Peggy Farnham
Lab: Michael Snyder, Stanford
Grant: U54HG004558
Download: human_TCF7L2_validation_Farnham.pdf

Method: ChIP-seq comparison

not reviewed

Caption: Anti-TCF7L2 validation. A) Western blot analysis: 25ug of nuclear extracts from HepG2, HelaS3 or MCF7 cells were separated by SDS-PAGE and transferred to a nitrocellulose membrane for immunoblotting with 1:1,000 anti-TCF7L2 C9B9 antibody in 5% milk/TBST. Two predominant bands corresponding to known isoforms of TCF7L2 are detected. B) Immunoprecipitation-Western blot analysis of TCF7L2. TCF7L2 was precipitated from 100ug HCT116 nuclear extracts using 2 ug of two different anti-TCF7L2 antibodies, C9B9 or C48H11 and 2ug control rabbit IgG was used as a control. The eluates of the immunoprecipitation reactions were resolved by SDS-PAGE and blotted as in A). Both anti-TCF7L2 antibodies efficiently precipitated the two different TCF7L2 isoforms from these extracts.
Submitted by: Peggy Farnham
Lab: Michael Snyder, Stanford
Grant: U54HG004558
Download: human_TCF7L2_validation_Farnham.pdf