ENCAB000ANZ
Alternate accession: ENCAB000AQH
Antibody against Homo sapiens H4K20me1
Bos taurus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab9051
- Lot ID
- 519198
- Characterized targets
- H4K20me1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H4, mono methylated at K20.
- Aliases
- bradley-bernstein:PchAb 129-V
- External resources
Characterizations
H4K20me1 (Homo sapiens)
thymusthymus
Method: immunoblot
compliant
- Submitter comment
- This western blot demonstrates the antibody recognizes a protein of the molecular weight consistent with human histone H3
- Reviewer comment
- Though this western blot was carried out with calf biosamples, the ENCODE Antibody Review Panel has reviewed this characterization and allowed this lot to be exempted for use in human biosamples on November 11, 2016.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H4K20me1 (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- Dot Blot Fabrication: Suitably modified peptides modeled on histone tails were obtained from Abcam. Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad) Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray. Place on rocker for 30 minutes. Dump off SuperBlock.
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570