Mus musculus
any cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Abcam
Product ID
ab8898
Lot ID
943257
Characterized targets
H3K9me3 (Mus musculus)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, tri methylated at K9.

Characterizations

H3K9me3 (Mus musculus)
Method: dot blot assay
not reviewed
Caption
Our validation point for mouse is that there is nothing species-specific about the existing validations. Synthetic peptides were used on the blot, and the assay was for specific reaction with the antibody. The peptides on the blot were not species-specific because HUMAN AND MOUSE HISTONE H3 ARE IDENTICAL IN THE RELEVANT REGIONS. Human and mouse H3 differ at only one position, amino acid 97, where a Cys in human is replaced by a Ser in mouse. There are NO differences in the relevant region, which is the N-terminal 36 amino acids.
Submitted by
Ross Hardison
Lab
Ross Hardison, PennState