ENCAB000ANY
Antibody against Mus musculus H3K9me3
Mus musculus
any cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab8898
- Lot ID
- 943257
- Characterized targets
- H3K9me3 (Mus musculus)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Isotype
- IgG
- Antigen description
- Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, tri methylated at K9.
- External resources
Characterizations
H3K9me3 (Mus musculus)
Method: dot blot assay
not reviewed
- Caption
- Our validation point for mouse is that there is nothing species-specific about the existing validations. Synthetic peptides were used on the blot, and the assay was for specific reaction with the antibody. The peptides on the blot were not species-specific because HUMAN AND MOUSE HISTONE H3 ARE IDENTICAL IN THE RELEVANT REGIONS. Human and mouse H3 differ at only one position, amino acid 97, where a Cys in human is replaced by a Ser in mouse. There are NO differences in the relevant region, which is the N-terminal 36 amino acids.
- Submitted by
- Ross Hardison
- Lab
- Ross Hardison, PennState
- Grant
- RC2HG005573