1. Antibodies
  2. Homo sapiens + Mus musculus
  3. HIST2H3A + HIST2H3D + HIST2H3C + Hist1h3d + Hist1h3b + Hist2h3c2 + Hist2h3c1 + Hist1h3e + Hist1h3f + Hist2h3b + Hist1h3c

ENCAB000ANU

Alternate accessions: ENCAB000ASE, ENCAB000ASF, ENCAB000AUQ

Antibody against Homo sapiens H3K4me3, Mus musculus H3K4me3

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Millipore
Product ID
07-473
Lot ID
Unknown
Host
rabbit
Clonality
polyclonal

Characterizations

H3K4me3 (Homo sapiens)
K562
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Western blot analysis of K562 lysate using rabbit polyclonal to H3K4me3. Submitting lab has confirmed with the vendor that lots R0903B0054, DAM1619901, DAM1623866, DAM1644057, DAM1651667, and DAM1661080 are identical.
Submitted by
Meital Hatan
Lab
Bradley Bernstein, Broad
H3K4me3 (Homo sapiens)
Method: dot blot assay
Attachment PDF Icon
compliant
Caption
Suitably modified peptides modeled on histone tails were obtained from Abcam.Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad) Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray.Place on rocker for 30 minutes. Dump off SuperBlock. Submitting lab has confirmed with the vendor that lots R0903B0054, DAM1619901, DAM1623866, DAM1644057, DAM1651667, and DAM1661080 are identical.
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
H3K4me3 (Mus musculus)
Method: dot blot assay
Attachment PDF Icon
not reviewed
Submitted by
Ross Hardison
Lab
Ross Hardison, PennState