ENCAB000ANM
Alternate accession: ENCAB000AQO
Antibody against Homo sapiens H3K9ac, Mus musculus H3K9ac
Mus musculus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Active Motif
- Product ID
- 39137
- Lot ID
- 1008001
- Characterized targets
- H3K9ac (Homo sapiens), H3K9ac (Mus musculus)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- crude
- Isotype
- serum
- Antigen description
- Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of histone H3
- Aliases
- bing-ren:19.3
- External resources
Characterizations
H3K9ac (Mus musculus)
Method: dot blot assay
compliant
- Caption
- Performed dot blot analysis using a large set of modified peptides.The only peptide recognized by the H3K9Ac antibody was the correct one (noted with #1).
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_19.3_dotblot.jpeg
H3K9ac (Mus musculus)
TT2
Method: immunoblot
compliant
- Caption
- An immunoblot using whole cell extract of two human cell lines and one mouse ES cell line shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_19.3_Western.jpg
H3K9ac (Homo sapiens)
HCT116HEK293
Method: immunoblot
compliant
- Caption
- An immunoblot using whole cell extract of two human cell lines and one mouse ES cell line shows positive bands at the size of the histone (17kDa), while the recombinant unmodified version of that histone shows a lack of reactivity. The histone bands detected in the extracts constitute 100% of the protein signal and show at least 10-fold enrichment relative to any other band. This signal is also at least 10-fold enriched relative to that detected using the unmodified recombinant histone.
- Submitted by
- Ah Young Lee
- Lab
- Bing Ren, UCSD
- Grant
- U54HG006997
- Download
- bing-ren_19.3_Western.jpg
H3K9ac (Mus musculus)
Method: dot blot assay
not compliant
- Caption
- Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K9Ac antibody was the correct one.
- Submitted by
- Yin Shen
- Lab
- Bing Ren, UCSD
- Grant
- R01HG003991
H3K9ac (Mus musculus)
TT2
Method: immunoblot
not compliant
- Submitted by
- Yin Shen
- Lab
- Bing Ren, UCSD
- Grant
- R01HG003991