ENCAB000ANK
Alternate accessions: ENCAB000AOW, ENCAB000AOV
Antibody against Homo sapiens H3K9ac
Homo sapiens
any cell type or tissue
characterized to standards
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab4441
- Lot ID
- 455103
- Characterized targets
- H3K9ac (Homo sapiens)
- Lot ID aliases
- 550799
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- Protein A
- Isotype
- IgG
- Antigen description
- Synthetic peptide: ARTKQTARAcKSTG-C conjugated to KLH, corresponding to amino acids 1-12 of Human Histone H3.
- Aliases
- bradley-bernstein:PchAb 116-V
- External resources
Characterizations
H3K9ac (Homo sapiens)
Method: dot blot assay
compliant
- Caption
- Dot blot assay reactivity of test antibody human H3K9ac with control peptides and singly modified peptides. Lot #550799 performed by Bernstein lab.
- Submitted by
- Marcus Ho
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K9ac (Homo sapiens)
K562HepG2GM12878
Method: immunoblot
compliant
- Caption
- 0.7ug unmodified Recombinant Histone H3 (Millipore 14-494) and 10ug of K562, GM12787, Huvec and SK-N-SH Whole Cell Lysate were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min.
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
H3K9ac (Homo sapiens)
Method: immunoblot
not reviewed
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
H3K9ac (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
- Caption
- Antibody was validated by analysis of ChIP-Seq data comparing tracks derived using the antibody in ChIPs performed on different cell types.
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570