1. Antibodies
  2. Homo sapiens
  3. HIST2H3A + HIST2H3D + HIST2H3C

ENCAB000ANF

Alternate accession: ENCAB000APK

Antibody against Homo sapiens H3K4me2

Bos taurus
any cell type or tissue
characterized to standards
Homo sapiens
any cell type or tissue
characterized to standards with exemption
Status
released
Source (vendor)
Abcam
Product ID
ab7766
Lot ID
56293
Characterized targets
H3K4me2 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, di methylated at K4.
Aliases
bradley-bernstein:PchAb 87-V

Characterizations

H3K4me2 (Homo sapiens)
thymusthymus
Method: immunoblot
Attachment PDF Icon
compliant
Submitter comment
This western blot demonstrates the antibody recognizes a protein of the molecular weight consistent with human histone H3
Reviewer comment
Though this western blot was carried out with calf biosamples, the ENCODE Antibody Review Panel has reviewed this characterization and allowed this lot to be exempted for use in human biosamples on November 11, 2016.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
H3K4me2 (Homo sapiens)
Method: dot blot assay
Attachment PDF Icon
compliant
Caption
Suitably modified peptides modeled on histone tails were obtained from Abcam. Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad) Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray. Place on rocker for 30 minutes. Dump off SuperBlock. Submitting lab has confirmed with the vendor that lots R0903B0054, DAM1619901, DAM1623866, DAM1644057, DAM1651667, and DAM1661080 are identical.
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad