ENCAB000AMZ
Antibody against Homo sapiens TFAP2C
Homo sapiens
any cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-8977
- Lot ID
- G1404
- Characterized targets
- TFAP2C (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Antigen description
- Raised against amino acids 145-221 mapping with in an internal region of AP-2Y of human origin.
- External resources
Characterizations
TFAP2C (Homo sapiens)
Method: knockdown or knockout
not reviewed
- Caption
- Depletion of AP2gamma following transfection of 100nM AP2gamma siRNA into MCF7 cells for 48Hrs. 100nM of siGlo was used in parallel as the control siRNA. 20ugs of whole cell extract were loaded for the Western blot, and the expected AP2gamma band was detected at 48 Kda with the anti-AP2gamma antibody (indicated by the red arrow). AP2gamma antibody: Santa Cruz Biotechnology rabbit polyclonal anti-AP2gamma (H-77) sc-8977
- Submitted by
- Peggy Farnham
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
TFAP2C (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
- Caption
- ChIP analysis of AP2alpha (AP2a) and AP2gamma (AP2Y) in MCF7 cells transfected with either 100nM of siAP2alpha or 100nM siGlo control for 72Hrs. The AP2gamma, but not the AP2alpha, ChIP signal on the Myc promoter is speci!cally reduced in MCF7 cells transfected with siAP2gamma compared to cells transfected with siGlo control siRNA. ChIP analysis of AP2alpha (AP2a) and AP2gamma (AP2Y) in MCF7 cells transfected with either 100nM of siAP2alpha or 100nM siGlo control for 72Hrs. The AP2gamma, but not the AP2alpha, ChIP signal on the Myc promoter is speci!cally reduced in MCF7 cells transfected with siAP2gamma compared to cells transfected with siGlo control siRNA. AP2gamma antibody: Santa Cruz Biotechnology rabbit polyclonal anti-AP2gamma (H-77) sc-8977
- Submitted by
- Peggy Farnham
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558