1. Antibodies
  2. Homo sapiens
  3. TFAP2A

ENCAB000AMY

Antibody against Homo sapiens TFAP2A

Homo sapiens
any cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-8975
Lot ID
E0804
Characterized targets
TFAP2A (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
Raised against amino acids 130-209 mapping within an internal region of AP-2alpha of human origin.

Characterizations

TFAP2A (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
Attachment from submitters
not reviewed
Caption
Depletion of AP2alpha following transfection of 100nM AP2alpha siRNA into HeLaS3 cells for 48Hrs. 100nM of siGlo was used in parallel as the control siRNA. 20ugs of whole cell extract were loaded for the Western blot, and the expected AP2alpha band was detected at 48 KDa with the anti-AP2alpha antibody (indicated by the red arrow). Depletion of AP2alpha following transfection of 100nM AP2alpha siRNA into HeLaS3 cells for 48Hrs. 100nM of siGlo was used in parallel as the control siRNA. 20ugs of whole cell extract were loaded for the Western blot, and the expected AP2alpha band was detected at 48 KDa with the anti-AP2alpha antibody (indicated by the red arrow). AP2alpha antibody: Santa Cruz Biotechnology rabbit polyclonal anti-AP2alpha (H-79) sc-8975
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
TFAP2A (Homo sapiens)
Method: ChIP-seq comparison
Attachment from submitter
Attachment from submitters
not reviewed
Caption
ChIP analysis of AP2alpha (AP2a) and AP2gamma (AP2Y) in MCF7 cells transfected with either 100nM of siAP2alpha or 100nM siGlo control for 72Hrs. The AP2alpha, but not the AP2gamma, ChIP signal on the Myc promoter is speci!cally reduced in MCF7 cells transfected with siAP2alpha compared to cells transfected with siGlo control siRNA.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford