ENCAB000AMC
Antibody against Homo sapiens TRIM37
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-49548
- Lot ID
- F2507
- Characterized targets
- TRIM37 (Homo sapiens)
- Host
- goat
- Clonality
- polyclonal
- Isotype
- IgG
- Antigen description
- Raised against a peptide mapping at the C-terminus of TRIM37 of human origin.
- External resources
Characterizations
TRIM37 (Homo sapiens)
not reviewed
- Caption
- IP followed by mass spectrometry: Briefly, HeLa whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. Gel was stained with Coomassie Blue in order to visualize marker bands. A gel fragment corresponding to the band indicated above in the western blot image was excised and sent to the University of Alabama at Birmingham Cancer Center Mass Spectrometry/Proteomics Shared Facility. There the sample was run on an LTQ XL Linear Ion Trap Mass Spectrometer by LC-ESI-MS/MS. Peptides were identified using SEQUEST tandem mass spectra analysis, with probability based matching at p < 0.05. As per ENCODE data standards, all SEQUEST results are attached, including common contaminants. Target protein is listed as hit 9a. Results in document "ENCODE_HAIB_TRIM37_(SC-49548)_03182011_MassSpec.pdf"
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576
TRIM37 (Homo sapiens)
not reviewed
- Caption
- Western blot protocol: Whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size visualized, representing strongest signal in the lane. Figure legend: IP-western with sc-49548 in whole cell lysates (WCL) of K562 and HeLa; PM=protein marker. TRIM37 band is indicated.
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576