1. Antibodies
  2. Homo sapiens
  3. TCF7L2

ENCAB000ALV

Antibody against Homo sapiens TCF7L2

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Cell Signaling
Product ID
2565
Lot ID
3
Characterized targets
TCF7L2 (Homo sapiens)
Host
rabbit
Clonality
monoclonal
Purification
affinity
Antigen description
Produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu81 of human TCF7L2.

Characterizations

TCF7L2 (Homo sapiens)
Method: immunoprecipitation
Attachment PDF Icon
not reviewed
Caption
TCF7L2 ChIP-seq peak overlap comparison. Two different TCF7L2 ChIP-seq datasets were prepared from HelaS3 cells either using the anti-TCF7L2 C9B9 antibody or the C48H11 antibody. Peaks were called using Solesearch peak calling program and the lists were compared directly.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
TCF7L2 (Homo sapiens)
Method: ChIP-seq comparison
Attachment PDF Icon
not reviewed
Caption
Anti-TCF7L2 validation. A) Western blot analysis: 25ug of nuclear extracts from HepG2, HelaS3 or MCF7 cells were separated by SDS-PAGE and transferred to a nitrocellulose membrane for immunoblotting with 1:1,000 anti-TCF7L2 C9B9 antibody in 5% milk/TBST. Two predominant bands corresponding to known isoforms of TCF7L2 are detected. B) Immunoprecipitation-Western blot analysis of TCF7L2. TCF7L2 was precipitated from 100ug HCT116 nuclear extracts using 2 ug of two different anti-TCF7L2 antibodies, C9B9 or C48H11 and 2ug control rabbit IgG was used as a control. The eluates of the immunoprecipitation reactions were resolved by SDS-PAGE and blotted as in A). Both anti-TCF7L2 antibodies efficiently precipitated the two different TCF7L2 isoforms from these extracts.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford