ENCAB000ALU

Antibody against Homo sapiens TCF3

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-349
Lot ID
A1111
Characterized targets
TCF3 (Homo sapiens)
Lot ID aliases
D2611
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Raised against a peptide mapping at the C-terminus of E2A.E12 of human origin.

Characterizations

TCF3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
not reviewed
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
TCF3 (Homo sapiens)
Method: immunoblot
not reviewed
Caption
Western blot protocol: Whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size (~67 kDa) visualized, representing strongest signal in the lane. Figure legend: IP-western with sc-349 in whole cell lysate (WCL) of K562; PM=protein marker. TCF3 band is indicated.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB