ENCAB000ALU
Antibody against Homo sapiens TCF3
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-349
- Lot ID
- A1111
- Characterized targets
- TCF3 (Homo sapiens)
- Lot ID aliases
- D2611
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Raised against a peptide mapping at the C-terminus of E2A.E12 of human origin.
- External resources
Characterizations
TCF3 (Homo sapiens)
not reviewed
- Caption
- Western blot protocol: Whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size (~67 kDa) visualized, representing strongest signal in the lane. Figure legend: IP-western with sc-349 in whole cell lysate (WCL) of K562; PM=protein marker. TCF3 band is indicated.
- Submitted by
- Richard Myers
- Lab
- Richard Myers, HAIB
- Grant
- U54HG004576