ENCAB000ALO

Antibody against Homo sapiens TAL1, Mus musculus TAL1

Homo sapiens
at least one cell type or tissue, G1E, G1E-ER4, MEL cell line, CH12.LX, K562
not characterized to standards
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-12984
Lot ID
J2209
Lot ID aliases
B2511
Host
goat
Clonality
polyclonal
Purification
affinity
Antigen description
Epitope mapping at the C-terminus of TAL1 of human origin.

Characterizations

TAL1 (Homo sapiens)
Method: immunoprecipitation
not reviewed
Caption
A single major band is observed in K562 nuclear lysates. This band is efficiently and specifically immunoprecipitated. While this band migrates somewhat slower than expected (expected molecular weight of ~34kD), we note that this behavior has been reported (see product characterization literature for this antibody and others against TAL1 on manufacturer'€™s website) and that the identity of this band was confirmed by mass spectrometry (see validation #2). Therefore, sc12984 meets this criterion for validation.
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
TAL1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
TAL1 (Mus musculus)
G1EG1E-ER4MEL cell lineCH12.LX
Method: immunoblot
compliant
Submitted by
Ross Hardison
Lab
Ross Hardison, PennState
TAL1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not reviewed
Caption
Immunoprecipitation of TAL1 from K562 cells using sc12984. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with ab87525. Lane 3: material immunoprecipitated using control IgG. Bands A and Be were excised from the gel and subject to analysis by mass spectrometry.
Reviewer comment
The review of this IP is dependent on the interpretation of resulting mass spec result since another TF was detected to be more enriched than TAL1.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
TAL1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
not compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using sc12984, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were trysinized using the in-gel digestion method. Digested proteins were analyzed on an LTQ-Orbitrap (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Protein false discovery rate < 1%, 2 peptides per protein minimum).
Submitter comment
Minyi Shi: I could not find any evidence of an interaction between ILF2 and TAL1
Reviewer comment
ILF2 (http://www.genecards.org/cgi-bin/carddisp.pl?gene=ILF2) is ranked higher than the target TF by enrichment, is ILF2 a known interacting partner of TAL1?
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download