ENCAB000ALJ

Homo sapiens

at least one cell type or tissue

awaiting characterization

Method: ChIP-seq comparison

not reviewed

Caption: (B) Knockdown of Suz12 using siRNAs demonstrates that the single band detected by Western blot is SUZ12. Western blot analysis of whole cell extracts prepared from the indicated cells after a 6-day transfection with SUZ12 siRNA, GFP siRNA, or no siRNA (Mock). The levels of actin are shown to demonstrate approximately equal loading of protein samples. As expected, since SUZ12 is a component of PRC2, the H3K27me3 signal was also greatly reduced. However, H3K9me3 levels were unchanged. See Squazzo et al. Genome Res. 2006 Jul;16(7):890-900.
Submitted by: Peggy Farnham
Lab: Michael Snyder, Stanford
Grant: U54HG004558
Download: human_SUZ12_validation_Farnham.pdf

Method: immunoblot

not reviewed

Caption: SUZ12 is a component of the PRC2 complex, which also contains the histone methyltransferase EZH2 that can methylate histone H3 on lysine 27. ChIP-seq analysis was performed in Ntera2 cells using an antibody to SUZ12 and to H3K27me3; two replicates of each were performed. The overlap of the SUZ12 replicates was 99% and the overlap of the H3K27me3 replicates was 98%. The peak sets of the merged SUZ12 and the merged H3K27me3 datasets were then overlapped. 80% (8419) of the SUZ12 called peaks were found within the H3K27me3 called peaks. [It should be noted that a smaller percentage of the H3K27me3 sites will overlap with SUZ12 sites because the H3K27me3 mark spreads over longer distances than the SUZ12 mark.]
Submitted by: Peggy Farnham
Lab: Michael Snyder, Stanford
Grant: U54HG004558
Download: human_SUZ12_validation_Farnham.pdf