1. Antibodies
  2. Homo sapiens
  3. STAT3

ENCAB000ALH

Antibody against Homo sapiens STAT3

Homo sapiens
at least one cell type or tissue
awaiting characterization
Homo sapiens
K562
partially characterized
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-482
Lot ID
B0111
Characterized targets
STAT3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Epitope mapping at the C-terminus of Stat3 of mouse origin.

Characterizations

STAT3 (Homo sapiens)
Method: immunoprecipitation
Attachment PDF Icon
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
STAT3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
STAT3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not submitted for review by lab
Caption
Briefly, STAT3 was immunoprecipitated from K562 whole cell lysates using antibody sc-482. A negative control IP using rabbit IgG was carried out in parallel and processed identically as follows. The immunoprecipitated fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated above in the Western blot image were excised and destained using the Pierce SilverSNAP Stain for Mass Spectrometry kit. Proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on an LTQ-Orbitrap (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Protein false discovery rate < 1%, 2 peptides per protein minimum). A schematic of the approximate location of the excised bands is shown in the figure and all proteins identified in these bands are shown in the table along with the ratio of peptides identified in the sc-482/Stat3 IP to peptides in the control IP. Stat3 is highlighted.
Submitter comment
We withdraw this characterization from review as we think it does not meet the required standard.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
STAT3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
STAT3 was immunoprecipitated from K562 whole cell lysates using antibody sc-482. Lane 1: Input lysate. Lane 2: Bound material from IP. Lane 3: Bound material from IP using non-specific IgG.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
 STAT3_IP_MS.png