ENCAB000ALG
Antibody against Homo sapiens STAT2
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Santa Cruz Biotech
- Product ID
- sc-476
- Lot ID
- B1908
- Characterized targets
- STAT2 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Peptide mapping at c-terminus of Human STAT2 p-113 (C-20) X
- External resources
Characterizations
STAT2 (Homo sapiens)
Method: immunoprecipitation
not reviewed
- Caption
- A single band consistent with the size expected for STAT2 (~113kD, indicated by arrow) is observed in material immunoprecipitated from HeLa S3 lysates using sc-476. This band is efficiently and specifically immunoprecipitated. Therefore, sc-476 meets this criterion for validation.
- Submitted by
- Michael Snyder
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
- Download
- human_STAT2_validation_Snyder.pdf
STAT2 (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
- Caption
- K562 cells treated with Interferon-α for the indicated times were used for ChIP-seq with antibody sc-345 (STAT1) or antibody sc-476 (STAT2). Peaks were called from replicate experiments using PeakSeq with a .01 q-value. Comparisons between experiments were made according to standard ENCODE replicate comparison parameters ( http://genome.ucsc.edu/ENCODE/protocols/dataStandards/ChIP_DNase_FAIRE_DNAme_v2_2011.pdf; reported above is the fraction of the top 40% of STAT1 peaks that are found in the full list of STAT2 peaks. The reciprocal comparison was made with similar results.).
- Submitted by
- Michael Snyder
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
- Download
- human_STAT2_validation_Snyder.pdf