at least one cell type or tissue
SP4 (Homo sapiens)
- For an antibody to meet ENCODE validation standards, a single band of the predicted size, or a band of no less than half the total signal, must be detected in a lane on a Western blot. Figure Legend: Western blot analysis of SP4 expression in NIH/3T3 (A,B) and KNRK (C,D) nuclear extracts. Western blot protocol Whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Figure Legend: SP4 immunoblot: IP-western with sc-645 SP4 antibody in whole cell lysates (WCL) of GM12878 and HeLa. Heavy chain and light chain of IgG are indicated, and SP4 band is indicated at ~120 kDa.
- Submitted by
- Richard Myers
- Richard Myers, HAIB