ENCAB000AKX

Antibody against Homo sapiens SMC3, Mus musculus SMC3

Homo sapiens
GM12878, K562, HeLa-S3, HepG2, neural cell, A549, IMR-90
characterized to standards with exemption
Homo sapiens
any cell type or tissue
partially characterized
Mus musculus
any cell type or tissue
Status
released
Source (vendor)
Abcam
Product ID
ab9263
Lot ID
963667
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Raised against synthetic peptide representing a portion within the last 100 amino acids of the human Structural Maintenance of Chromosomes-3 conjugated to KLH.

Characterizations

SMC3 (Homo sapiens)
Method: knockdown or knockout
Attachment from submitter
exempt from standards
Caption
A new secondary characterization is not required as one exists for ENCAB749XQY which is a lot of the same antibody (ab9263). The primary characterization of K562 and HepG2 for this lot (https://www.encodeproject.org/antibody-characterizations/3520cc2e-c067-4df0-9d68-7f61744a75f5/) shows the same banding pattern as the primary characterization (https://www.encodeproject.org/antibody-characterizations/b1ccde77-1ec6-4eb3-8930-2dd9fe002bcd/ https://www.encodeproject.org/antibody-characterizations/a8ff6fa7-7588-4417-b7d4-869333e1d381/) for the same respective cell lines for the lot ENCAB749XQY.
Submitter comment
This is a new lot of a previously characterized antibody ENCAB749XQY and we think no further characterization is necessary beyond the primary if the banding pattern is consistent with the old.
Reviewer comment
The banding pattern in K562 and HepG2 is consistent with that of the primary characterizations associated with old lot ENCAB749XQY so a new secondary characterization for the new lot is not needed.
Submitted by
Esther Chan
Lab
Michael Snyder, Stanford
SMC3 (Homo sapiens)
A549IMR-90
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
New primary characterizations for A549 and IMR-90 are not required as the banding pattern for characterized cell lines in common (K562 and HepG2) with another fully characterized lot ENCAB749XQY of the same antibody (ab9263). Primary characterizations of ENCAB749XQY in A549 and IMR-90 also exhibit the same banding pattern.
Submitter comment
We think this antibody lot should be exempted from primary characterization in A549 and IMR-90 as we've demonstrated that the lot behaves the same in immunoprecipitations as another lot (ENCAB749XQY) in those and other cell types.
Reviewer comment
This antibody was exempted from primary characterization in A549 and IMR-90 by the ENCODE antibody review panel on October 17, 2016.
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford
SMC3 (Homo sapiens)
neural cell
Method: immunoprecipitation
Attachment from submitter
exempt from standards
Caption
The ENCODE antibody standards document exempts some valuable and/or limiting samples from primary characterizations for well-characterized antibodies. They are given exemptions so that the samples can be conserved to carry out the downstream experiments.
Submitter comment
We do not have any of the H1-derived neurons that were distributed to the consortium for analysis in ENCODE2 left.
Reviewer comment
This cell type was exempted from primary characterization of antibody ENCAB000AKX by the ENCODE antibody review panel on October 17, 2016
Submitted by
Jessika Adrian
Lab
Michael Snyder, Stanford
SMC3 (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
Attachment from submitter
compliant
Caption
A.  Western blot using ab9263. Lanes contain nuclear lysates from GM12878 cells (Lane 1), K562 cells (Lane 2), HeLa S3 cells (Lane 3), and HepG2 cells (Lane 4).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
SMC3 (Homo sapiens)
Method: immunoprecipitation
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
SMC3 (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
SMC3 (Mus musculus)
Method: ChIP-seq comparison
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
SMC3 (Mus musculus)
Method: immunoprecipitation
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford