ENCAB000AKP

Antibody against Homo sapiens SETDB1

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Proteintech
Product ID
11231-1-AP
Lot ID
002
Characterized targets
SETDB1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity

Characterizations

SETDB1 (Homo sapiens)
Method: immunoblot
not reviewed
Caption
SETDB1 antibody validation 1: Immunoprecipitation-western blot SETDB1 was immunoprecipitated from K562 nuclear extracts using two different antibodies and run on SDS-PAGE for western blotting using the Proteintech group anti-SETDB1 antibody (at a concentration of 1ug/mL or 1:1,000). 2ug of either the Proteintech group or Santacruz anti-SETDB1 antibodies were used and 2ug of rabbit non-specific IgGs were used as a control and compared to 1% of input nuclear extract. Both Proteintech group and Santacruz anti-SETDB1 antibodies precipitated a protein of the corresponding molecular weight of SETDB1, while the control IgGs did not.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
SETDB1 (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
Caption
SETDB1 antibody validation 2: ChIP-seq peak overlap between two different anti-SETDB1 antibodies. SETDB1 ChIP-seq experiments were performed in K562 cells with two different anti-SETDB1 antibodies, Proteintech group (PTG) or Santacruz (H-300). Peaks were called using Sole-search and the overlap between the peak lists were performed. Greater than 80% of the top 40% of the peaks identified using the PTG anti-SETDB1 antibody were present in the list of H-300 peaks and vice versa.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford