ENCAB000AKO
Antibody against Homo sapiens SAP30
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
K562, HepG2, GM12878, SK-N-SH
not characterized to standards
- Status
- released
- Source (vendor)
- Active Motif
- Product ID
- 39731
- Lot ID
- 4710001
- Characterized targets
- SAP30 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- crude
- Antigen description
- Raised against recombinant full length human SAP30 protein.
- Aliases
- bradley-bernstein:PchAb 164-V
- External resources
Characterizations
SAP30 (Homo sapiens)
not reviewed
- Caption
- Antibody was validated by use of correlation analysis of ChIP-String and ChIP-Seq data
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
- Download
- human_SAP30_validation_Bernstein.pdf
SAP30 (Homo sapiens)
not reviewed
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
- Download
- human_SAP30_validation_Bernstein.pdf
SAP30 (Homo sapiens)
K562HepG2GM12878SK-N-SH
not compliant
- Reviewer comment
- KCO: None of the major bands shown on the gel are within 20% of the expected band size (30 kDa).
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
SAP30 (Homo sapiens)
K562
not compliant
- Caption
- 0.25ug unmodified Recombinant Histone H3 (Millipore 14-494) and 30ug of K562 Whole Cell Lysate (Abcam, ab7911) were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and WCL, at similar intensities.Panel B: Band of expected size (~17kDa) visualized in WCL, and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since no reactivity with nonhistone proteins or unmodified histones was detected
- Reviewer comment
- KCO: Image (and caption) neither provides the cell line used, nor the precise expected band size value (based on Antibody Characterization Standards, August 2015)
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
SAP30 (Homo sapiens)
compliant
- Caption
- This validation relies on the use of antibodies to different members of a known complex, in K562 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The second track shown used an antibody to HDAC1 (PchAb 134-V), another member of the Sin3a complex. Overall correlation score: SAP30 vs. HDAC1: 0.9303
- Reviewer comment
- Currently under "pending dcc review" because the document needs to include the accession of the antibody used for comparison, rather than just an internal identifier.
- Submitted by
- Kathrina Onate
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- SAP30_PchAb_164-V_WIP_SAV.pdf