1. Antibodies
  2. Homo sapiens
  3. PML

ENCAB000AKA

Antibody against Homo sapiens PML

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-71910
Lot ID
H1908
Characterized targets
PML (Homo sapiens)
Host
mouse
Clonality
monoclonal
Isotype
IgG
Antigen description
N-terminal epitope corresponding to residues 37-51 of PML of human origin.

Characterizations

PML (Homo sapiens)
Method: immunoblot
Attachment PDF Icon
not reviewed
Caption
Western blot protocol: Whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Bands of expected size visualized, representing strongest signal in the lane. Both bands tested by IP-mass spec. Figure legend: IP-western with sc-71910 in whole cell lysate (WCL) of GM12878 and HeLa; PM=protein marker. PML bands are indicated.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
PML (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
not reviewed
Caption
IP followed by mass spectrometry: Briefly, GM12878 whole cell lysates were immunoprecipitated using primary antibody, and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. Gel was stained with Coomassie Blue in order to visualize marker bands. Gel fragments corresponding to the bands indicated above in the western blot image were excised and sent to the University of Alabama at Birmingham Cancer Center Mass Spectrometry/Proteomics Shared Facility. There the samples were run on an LTQ XL Linear Ion Trap Mass Spectrometer by LC-ESI-MS/MS. Peptides were identified using SEQUEST tandem mass spectra analysis, with probability based matching at p < 0.05. As per ENCODE data standards, all SEQUEST results are attached(ENCODE_HAIB_PML_sc71910_09012011_MassSpec.pdf), including common contaminants. Target protein is listed as hit 10a in lower (~80 kDa) band and hit 6a in upper (~100 kDa) band.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB