ENCAB000AJX
Antibody against Homo sapiens PHF8
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
K562
not characterized to standards
- Status
- released
- Source (vendor)
- Bethyl Labs
- Product ID
- A301-772A
- Lot ID
- 1
- Characterized targets
- PHF8 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Aliases
- bradley-bernstein:PchAb 377
- External resources
Characterizations
PHF8 (Homo sapiens)
Method: immunoblot
not reviewed
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
- Download
- human_PHF8_validation_Bernstein.pdf
PHF8 (Homo sapiens)
Method: ChIP-string comparison
not reviewed
- Caption
- Antibody was validated by use of correlation analysis of ChIP-String and ChIP-Seq data
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
- Download
- human_PHF8_validation_Bernstein.pdf
PHF8 (Homo sapiens)
Method: ChIP-seq comparison
compliant
- Caption
- This validation relies on the use of antibodies to a chromatin regulator and a functionally related histone modification in K562 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The first track shown used an antibody to PHF8 (PchAb 377, ENCAB000AJX), and the second track shown used an antibody to H3K4me3 previously characterized to Encode standards (PchAb 99-V, ENCAB000BLE).
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- PHF8 PchAb 377 SAV.pdf
PHF8 (Homo sapiens)
K562
Method: immunoblot
not compliant
- Reviewer comment
- KCO: Image (and caption) neither provides the cell line used, nor the precise expected band size value
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
PHF8 (Homo sapiens)
K562K562
Method: immunoprecipitation
not compliant
- Caption
- K562 whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction along side a whole cell lysate were loaded on a CriterionXT gel and separated. After separation, the samples were transferred using a wet transfer. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed. Band of expected size visualized representing strongest signal in the lane.
- Reviewer comment
- needs IgG
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- phf8_bethyl_A310-772A-1_IP.png