ENCAB000AJX

Antibody against Homo sapiens PHF8

Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
K562
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-772A
Lot ID
1
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
bradley-bernstein:PchAb 377

Characterizations

PHF8 (Homo sapiens)
Method: immunoblot
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
PHF8 (Homo sapiens)
Method: ChIP-string comparison
not reviewed
Caption
Antibody was validated by use of correlation analysis of ChIP-String and ChIP-Seq data
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
PHF8 (Homo sapiens)
Method: ChIP-seq comparison
compliant
Caption
This validation relies on the use of antibodies to a chromatin regulator and a functionally related histone modification in K562 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. The first track shown used an antibody to PHF8 (PchAb 377, ENCAB000AJX), and the second track shown used an antibody to H3K4me3 previously characterized to Encode standards (PchAb 99-V, ENCAB000BLE).
Submitted by
Nina Farrell
Lab
Bradley Bernstein, Broad
PHF8 (Homo sapiens)
K562
Method: immunoblot
Attachment from submitter
not compliant
Reviewer comment
KCO: Image (and caption) neither provides the cell line used, nor the precise expected band size value
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
PHF8 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
K562 whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction along side a whole cell lysate were loaded on a CriterionXT gel and separated. After separation, the samples were transferred using a wet transfer. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed. Band of expected size visualized representing strongest signal in the lane.
Reviewer comment
needs IgG
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad