ENCAB000AJV

Alternate accession: ENCAB799JRJ

Antibody against Homo sapiens KAT2B

Homo sapiens
at least one cell type or tissue
awaiting characterization
Homo sapiens
A549, K562, H1-hESC
not characterized to standards
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-666A
Lot ID
1
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
bradley-bernstein:PchAb 154

Characterizations

KAT2B (Homo sapiens)
Method: ChIP-string comparison
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
KAT2B (Homo sapiens)
Method: immunoblot
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
KAT2B (Homo sapiens)
A549H1-hESC
Method: immunoblot
Attachment from submitter
not compliant
Caption
Nuclear lysates from A549 (10ug) and H1 (10ug) were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Bands were detected in a range below the expected size (110kDa).
Reviewer comment
Band intensity is not 50% of total signal for both lanes
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
KAT2B (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
K562 whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction along side a whole cell lysate were loaded on a CriterionXT gel and separated. After separation, the samples were transferred using a wet transfer. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed. Band of expected size visualized representing strongest signal in the lane.
Reviewer comment
No IgG control
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad