at least one cell type or tissue
PBX3 (Homo sapiens)
- Western blot protocol: Whole cell lysates were immunoprecipitated using primary antibody (sc-891), and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size visualized, representing strongest signal in the lane. Figure legend: IP-western with sc-891 in WCL (whole cell lysates) of GM12878 and HeLa; PM=protein marker. PBX3 band is indicated.
- Submitted by
- Richard Myers
- Richard Myers, HAIB