1. Antibodies
  2. Homo sapiens
  3. NFYA

ENCAB000AJC

Antibody against Homo sapiens NFYA

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Roberto Mantovani
Product ID
NF-YA
Lot ID
unknown
Characterized targets
NFYA (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Antigen description
Raised against amino acids 139-347 of recombinant human NFYA.

Characterizations

NFYA (Homo sapiens)
Method: immunoprecipitation
Attachment PDF Icon
not reviewed
Caption
HeLaS3 nuclear extract (5x10^7cells/IP) was incubated with 10ug of anti-NFYA or normal rabbit IgG (SC-2027, Santa Cruz Biotechnology) and immunoprecipitated with protein A sepharose beads. Input nuclear extract, supernatant after IP and elution fractions were analysed by Western blotting as above, except: 10-14% gradient gel, 1:700 dilution anti-NFYA. Specific immunoprecipitation of the short and long NFYA isoforms was observed only in the anti-NFYA IP (indicated by arrows). Additional validation was carried out by observing specific ChIP-QPCR signals at known NFY bound genomic loci, and by comparison to NFYB ChIP-Seq data showing high degree of correlation between NFYA and NFYB read counts at NFY bound regions across 3 cell lines (Pearson correlation >0.7).
Submitted by
Kevin Struhl
Lab
Michael Snyder, Stanford
NFYA (Homo sapiens)
Method: immunoblot
Attachment PDF Icon
not reviewed
Caption
Nuclear extract, of the indicated cell lines, were separated on a 10% gel using SDS-PAGE, transferred to a PDF membrane and Western blotted using standard protocols. Primary antibody (anti-NFYA, gift from Prof. R. Mantovani) and secondary antibody (SC-2317, Santa Cruz Biotechnology) were used at a dilution of 1:2500 and 1:4000, respectively. Signal was detected using Luminol Reagent (SC-2048, Santa Cruz Biotechnology). Detection of the known short and long NFYA isoforms are indicated by arrows.
Submitted by
Kevin Struhl
Lab
Michael Snyder, Stanford