ENCAB000AIZ

Antibody against Homo sapiens NELFE

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-32912
Lot ID
unknown
Characterized targets
NELFE (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
Protein A/G

Characterizations

NELFE (Homo sapiens)
Method: immunoblot
not reviewed
Submitted by
Kevin Struhl
Lab
Michael Snyder, Stanford
NELFE (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
Caption
Loaded image but it was not visible in window. Please contact me for image. Analysis of NELFe ChIP-Seq. confirms specificity of ChIP. A The 55% of MACS called NELFe ChIP-Seq peaks (p-value < 1e-5) were found within 500bp of a RefSeq TSS. The median peak position was 55 bp downstream of the TSS, consistent with the role of NELF in blocking RNA polymerase II after early elongation. B Comparison of NELFe MACS ChIP-Seq peaks to RNAPII, RNAPII (pS2), TFIIB, TBP and Spt5 ChIP-Seq peaks place NELFe coincident with partner negative elongation factor, DSIF (Spt5), and upstream of RNAPII, RNAPII(pS2), TBP and TFIIB.
Submitted by
Kevin Struhl
Lab
Michael Snyder, Stanford