1. Antibodies
  2. Homo sapiens
  3. MEF2C

ENCAB000AIR

Antibody against Homo sapiens MEF2C

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-13268
Lot ID
K2009
Characterized targets
MEF2C (Homo sapiens)
Host
goat
Clonality
polyclonal
Isotype
IgG
Antigen description
Epitope mapping near the C-terminus of MEF2C of human origin.

Characterizations

MEF2C (Homo sapiens)
Method: immunoblot
Attachment PDF Icon
not reviewed
Caption
Western blot protocol: Whole cell lysates were immunoprecipitated using primary antibody (SC-13268), and the IP fraction was loaded on a 12% acrylamide gel and separated with a Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed on an Invitrogen BenchPro 4100 system. Visualization was achieved using SuperSignal West Femto solution (Thermo Scientific). Results: Band of expected size visualized, representing strongest signal in the lane. Figure legend: IP-western with SC-13268 in WCL (whole cell lysates) of K562 and GM12878; PM=protein marker. MEF2C band is indicated.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB
MEF2C (Homo sapiens)
Method: motif enrichment
Attachment PDF Icon
not reviewed
Caption
Motif enrichment validation protocol: Motif enrichment validation was performed by Pouya Kheradpour. Methods and results are available at http://www.broadinstitute.org/~pouyak/encode-motif-disc/. Briefly, motif identification was performed on 100 bp peaks (50 bp on either side of peak summit), passing IDR between replicate experiments at < .01. Identified motifs were matched to known motifs for the tested TF (correlation > .75). ENCODE standards call for > 4-fold enrichment and > 10% motif representation in order to consider the identified motif validated. These standards are met and represented in the attached file, which includes cell line tested, name of maximally enriched motif, fold-enrichment value (as log2), and representation percentage. Additionally, known motif consensus sequences which were confirmed experimentally are displayed.
Submitted by
Richard Myers
Lab
Richard Myers, HAIB